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Purpose of the Experiment
The purpose of this experiment is to isolate two cultures. To isolate the two cultures, an isolation streak plate technique was used (Carolina Distance Learning 3). In a microbiology experiment, the isolation streak plate technique helps to isolate a pure culture from a mixed culture. The technique is important when there is a need to separate organisms in a mixed culture or isolate a given strain from contaminants (Carolina Distance Learning 3). In addition, the technique is used to grow a mixture of cultures on a growth media surface to allow an individual culture can be isolated and sampled. The lesson learned from this experiment is that it is possible to isolate two or more cultures mixed to get a pure culture. It is also possible to detect possible contamination of cultures.
Reflection
The use of the isolation streak plate technique to separate the two cultures was an interesting process to me. In this experiment, I contributed to the transfer of an older, reactivated culture to the fresh broth. I reviewed the video “transfer from broth to broth” to get some insights on how to effectively transfer culture to a fresh broth (Carolina Distance Learning 6). The video helped me a lot in making sure no mistake would occur.
After watching the video, I started the process of transferring the Micrococcus luteus to a new broth. I mixed the content of the culture tube by gently tapping it on the tubes using my palm. With the help of the video, I was able to aseptically transfer a loop of broth to a fresh tube containing nutrients to support the growth of the culture. Therefore, I effectively performed my tasks as provided in the guide.
Work Cited
Carolina Distance Learning. “Isolation streak plate technique.” Investigation Manual, 2018, pp. 1–12. Web.
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